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However, use of sperm centrifugation is detrimental to the sperm viability and elicits production of reactive oxygen species.

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Egg cells are often fertilized by sperm which would have low chance of fertilizing it in natural conditions.

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New flow-cytometry based techniques such as YO-PRO staining can discriminate apoptotic and dead spermatozoa from the viable ones.

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This selection is largely circumvented in case of in-vitro fertilization which leads to higher incidence of birth defects associated with assisted reproductive techniques.

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That is to say, there will always be some "male" sperm among the "female" sperm and vice versa.

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If applied large-scale, it has a potential to elicit a sex-ratio imbalance.

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The sperm is selected not only by its highest motility but also by other factors such as DNA integrityproduction of reactive oxygen species and viability.

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Utilizing artificial insemination with sorted sperm is seen as a way to create an optimal ratio of male and female calves to increase dairy milk production.

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Additionally, there is ongoing debate about using sperm sorting for choosing the child's sex.

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